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Towards Biomarkers for Improving Disease Resistance in Pigs
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  1. Introduction
  2. Slide 2
  3. Slide 3
  4. Area under Log Curve of shed Salmonella: A measure of disease spread potential
  5. Finding markers and classifiers for improving pork safety
  6. Slide 6
  7. Microarrays: measuring thousands of RNAs at once
  8. A “Gene Expression Signature” that predicts a biological outcome- like cancer survival
  9. “MammaPrint” classifier - a diagnostic test for breast cancer prognosis
  10. Slide 10
  11. Shedding phenotype: AULC in 40 challenged pigs
  12. Studying immune response to pathogen
  13. Slide 13
  14. Finding important response pathways: InnateDB analysis
  15. Slide 15
  16. Slide 16
  17. Slide 17
  18. Innate DB: pathways over-represented in genes DE post-infection in PS animals
  19. Sub-Network Enrichment Analysis- looking for regulators of major portions of the response
  20. SNEA Identifies Major Regulators of Genes in DE List
  21. Some regulators are themselves DE
  22. Some regulators are themselves DE
  23. Some regulators are themselves DE
  24. Some regulators are themselves DE
  25. Response to Sal using Q-PCR: miR-155 ? miR-155 targets ?
  26. Network of integrated pathways affected by Salmonella measured in whole blood RNA
  27. SNEA Identifies Major Regulators of Genes in DE List
  28. Slide 28
  29. Interferon gamma (IFNG)
  30. Using immune response to find tools to manage disease
  31. Can you use blood RNA response to predict shedding?
  32. Measurement of circulating IFN gamma levels in a second challenge confirms previous correlation
  33. Slide 33
  34. Are IFN gamma levels a major cause of gene expression patterns?
  35. Slide 33
  36. Are IFN gamma levels a major cause of gene expression patterns?
  37. Slide 35
  38. In vivo and in vitro treatments
  39. Slide 37
  40. Slide 38
  41. Slide 39
  42. Slide 40
  43. Studying immune response to pathogen versus expression patterns prior to infection
  47. Slide 45
  49. Slide 45
  50. Slide 46
  52. Slide 48
  53. Conclusions
  54. Future work- other relevant projects
  55. NF-?B Inhibitor: SC-514
  56. Transcriptional Mechanisms in Porcine Endotoxin response- Genes dependent on NF?B signaling
  57. Hierarchical clustering and annotation
  58. Future work- new SCID pig discovery
  59. Slide 55
  60. Questions?
00:00 / 00:00
CC
We were able to verify this observation in pig
blood in a second Salmonella challenge that I will describe in detail later. Both the
correlation of individual IFNgamma and sig diff between shedding
extremes was confirmed. So we are interested in taking this further, and… Ok, just say the latter half Ok, just say the latter half